NK92MI（人恶性非霍奇金淋巴瘤患者的自然杀伤细胞）
CBP61113
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| I. General information | |
| Synonyms: | NK92MI |
| Background: | NK-92MI is an interleukin-2 (IL-2) independent Natural Killer Cell line derived from the NK-92 (ATCC CRL-2407) cell line by transfection. NK-92 is an interleukin-2 (IL-2) dependent Natural Killer Cell line derived from peripheral blood mononuclear cells from a 50 year old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. The parental cells were transfected with human IL-2 cDNA in the retroviral MFG-hIL-2 vector by particle-mediated gene transfer. The transfection is stable. |
| Species: | Homo sapiens, human |
| Tissue: | peripheral blood, blood |
| Disease: | malignant non-Hodgkin's lymphoma |
| Gender: | male, 50 years, Caucasian |
| Morphology: | lymphoblast |
| Growth Mode: | suspension, multicell aggregates |
| Doubling Time: | N/A |
| DNA Profile: | D5S818: 12. 13 D13S317: 9, 12 D7S820: 10, 11 D16S539: 11, 12 vWA: 16, 18 THO1: 6, 9.3 TPOX: 8 CSF1PO: 11, 12 Amelogenin: X, Y Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
75% MEM α (GIBCO 12561-056 ) + 12.5% FBS+12.5% horse serum + 0.2 mM inositoll+0.02 mM folic acid+0.05 mM 2-mercaptoethanol NK92MI完全培养基，# CBP61113M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | N/A |
| Receptor Expression: | N/A |
| Oncogene: | N/A |
| Genes Expressed: | N/A |
| Cellular Products: | N/A |
| Tumor Formation: | N/A |
| Comments: | NK-92 and this derivative cell line NK-92MI have the following characteristics: surface marker positive for CD2, CD7, CD11a, CD28, CD45, CD54 and CD56 bright; surface marker negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR. The parental IL-2 dependent cell line is available as ATCC CRL-2407 (NK-92). NK-92MI was shown to contain, express, and synthesize the hIL-2. A culture submitted to the ATCC in September of 1998 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative. ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR. For more information, please contact Cobioer (4008-750-250). |
