K562（人慢性粒细胞白血病细胞）
CBP60529
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| I. General information | |
| Synonyms: | K562 |
| Background: | The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises. |
| Species: | Homo sapiens, human |
| Tissue: | bone marrow |
| Disease: | leukemia |
| Gender: | female.53 yearss |
| Morphology: | lymphoblast |
| Growth Mode: | suspension |
| DNA Profile: | Amelogenin: X CSF1PO: 9,10 D13S317: 8 D16S539: 11,12 D5S818: 11,12 D7S820: 9,11 THO1: 9.3 TPOX: 8,9 vWA: 16 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS K562完全培养基，# CBP60529M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | CD7 (25%) |
| Tumor Formation: | Yes, in nude mice. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells |
| Comments: | The cell population has been characterized as highly undifferentiated and of the granulocytic series. Studies conducted by Anderson, et al., on the surface membrane properties led to the conclusion that the K-562 was a human erythroleukemia line. K-562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognizable progenitors of the erythrocytic, granulocytic and monocytic series.The effect of inducers on sublines derived from the original K-562 cell line have been reviewed by Koeffler and Golde.Karyological studies on various K-562 sublines have been classified into three groups (A,B,C) by Dimery, et al.The strain obtained by the ATCC most closely resembles the B population. Occurrence of the Philadelphia chromosome, however, was of much lower frequency; none detected in 15 metaphases examined.The line is EBNA negative. For more information, please contact Cobioer (4008-750-250). |
