MEC-1（人白血病细胞）
CBP60514
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| I. General information | |
| Synonyms: | MEC-1 |
| Background: | established in 1993 from the peripheral blood of a 61-year-old Caucasian man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL); serial sister cell line of MEC-2 (DSMZ ACC 500) |
| Species: | human (Homo sapiens) |
| Tissue: | N/A |
| Disease: | chronic B cell leukemia |
| Gender: | N/A |
| Morphology: | round to polymorphic cells growing in suspension, singly or partly in small aggregates, a few cells are slightly adherent; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 40 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
IMDM+20%FBS MEC-1完全培养基，# CBP60514M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +; image |
| Fingerprint: | same DNA profile as MEC-2 using multiplex PCR of minisatellite markers |
| Species: | confirmed originally as human by cytogenetics |
| Cytogenetics: | human near-diploid karyotype with 10% polyploidy - 46(44-47)<2n>XY, -2, +7, -12, +1-2mar, t(1;6)(q22-23;p21), add(7)(q11), der(10)(10pter->q22::?::2q11->qter), del(17)(p11) - small acf/mar present in most cells - resembles published karyotype |
| Virus | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
