U251（人脑胶质瘤细胞）
CBP60300
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| I. General information | |
| Synonyms: | U251 |
| Background: | Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (ECACC catalogue number 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. A new deposit of U-373 MG known as U-373 MG (Uppsala) is now available (ECACC catalogue number 08061901). Background to the identity query for the cell line U-373 MG: The American Type Culture Collection (ATCC) reported that their stock of U-373 MG had been shown to have differing genetic properties to stock from the originator’s laboratory, and to share similarities with another glioblastoma cell line, U-251. In light of this, ECACC undertook an investigation into the authenticity of its own stock of the U-373 MG cell line. ECACC found similar results to the ATCC i.e. the stock held as U-373 MG was found to be identical by STR-PCR profiling to U-251. The U-373 MG cell line listed under catalogue number 89081403 has been re-named as ‘U-251 MG (formerly known as U-373 MG)’ and has the new ECACC catalogue number 09063001. |
| Species: | human (Homo sapiens) |
| Tissue: | Brain |
| Disease: | Human glioblastoma astrocytoma |
| Gender: | N/A |
| Morphology: | Pleomorphic/astrocytoid |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 10,11 D16S539: 12 D5S818: 11,12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 16,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
MEM + 10% FBS + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) U251完全培养基，# CBP60300M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | 2n = 46 |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
