YAPC（人胰腺腺癌细胞）
CBP60698
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| I. General information | |
| Synonyms: | YAPC |
| Background: | established from ascites obtained during exploratory laparatomy of a 43-year-old Japanese man with pancreas carcinoma (terminal, no therapy) in December 1993; cells were described as being dependent on autocrinely secreted IL-1alpha |
| Species: | human (Homo sapiens) |
| Tissue: | pancreas |
| Disease: | pancreas carcinoma |
| Gender: | N/A |
| Morphology: | cobblestone-like adherent cells growing in monolayers; |
| Growth Mode: | N/A |
| Doubling Time: | ca. 48 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI1640+10%FBS YAPC完全培养基，# CBP60698M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 (+), cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+) |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human with IEF of AST, NP |
| Cytogenetics: | human near-triploid karyotype with 8% polyploidy - 66-71<3n>XXYY, +3, +7, +8, +9, +10, +14, -17, -18, -19, -20, -22, -22, -22, +3mar, der(1)del(1)(q23-25)inv(1)(p11q21), der(1;5)(q10;q10), add(3)(p11)/del(3)(p11p21)x2, del(5)(p11), add(6)(p21), del(7)(q32), add(9)(p1?), der(9;15)(q10;q10), der(10)t(10;11)(p11;q13)x2, del(12)(p12), add(13)(p11)x2, add(13)(p11), inv(16)(p13q11.2), del(18)(q21), add(20)(p11)/der(20)t(1;20)(p34;p11) - loss of 1p, 3p, 9p, 18q associated with pancreas carcinoma - matches published karyotype |
| Virus | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
