NCI-N87（人胃癌细胞）
CBP60491
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| I. General information | |
| Synonyms: | NCI-N87 |
| Species: | Homo sapiens, human |
| Tissue: | stomach; derived from metastatic site: liver |
| Disease: | gastric carcinoma |
| Gender: | male |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 47 hrs |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 8,12 D13S317: 8,11 D16S539: 9,13 D5S818: 12,13 D7S820: 10,11 THO1: 9 TPOX: 9,11 vWA: 15,16 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI1640+10%FBS NCI-N87完全培养基，# CBP60491M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Receptor Expression: | acetylcholine, muscarinic, expressed ,Ref |
| Oncogene: | myc +; erb B2 + |
| Comments: | NCI-N87 cells express the surface glycoproteins carcinoembryonic antigen (CEA) and TAG 72, and are L-dopa decarboxylase (DDC) negative. They were minimally positive for vasoactive intestinal peptide (VIP) receptors and lacked gastrin receptors. They were found to express receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted with the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of c-myc and c-erb-B 2 RNA that were comparable to other cell lines.There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. NCI-N87 cells have a reported plating efficiency of 4.3%. For more information, please contact Cobioer (4008-750-250). |
