MDA-MB-435S（人乳腺导管癌细胞（被黑色素细胞污染））
CBP60407
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| I. General information | |
| Synonyms: | MDA-MB-435S |
| Species: | Homo sapiens, human |
| Cell Type: | melanocyte |
| Disease: | Melanoma; Previously described as ductal carcinoma |
| Morphology: | spindle shaped |
| Growth Mode: | adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 13 D5S818: 12 D7S820: 8,10 THO1: 6,7 TPOX: 8,11 vWA: 16,18 Cobioer’s Cell Line Authentication Service |
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 2 PGM3, 1 |
| Culture Medium: |
DMEM + 10% FBS+10 ug/ml insulin+ 10 ug/ml glutathione MDA-MB-435S完全培养基，# CBP60407M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Antigen Expression: | N/A |
| Receptor Expression: | N/A |
| Oncogene: | N/A |
| Genes Expressed: | tubulin; actin |
| Cellular Products: | tubulin; actin |
| Tumor Formation: | No, in immunosuppressed mice Yes, in semisolid medium |
| Comments: | This cell line was originally described as a spindle shaped variant of the parental MDA-MB-435 strain isolated in 1976 by R. Cailleau, et al. from the pleural effusion of a 31 year old female with metastatic, ductal adenocarcinoma of the breast. However, recent studies have generated questions about the origin of the parent cell line, MDA-MB-435, and by extension HTB-129. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast [PubMed ID: 10700174, PubMed ID: 15150101, PubMed ID: 15679052]. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line [PubMed ID: 12354931 and PubMed ID: 17004106]. Derivatives of HTB-129 with identities in question: M4A4, ATCC® CRL-2914 M4A4 GFP, ATCC® CRL-2915 M4A4 LM3-2 GFP, ATCC® CRL-2916 M4A4 LM3-4 CL 16 GFP, ATCC® CRL-2917 NM2C5, ATCC® CRL-2918 NM2C5 GFP, ATCC® CRL-2919 For more information, please contact Cobioer (4008-750-250). |
