PC-3（人前列腺癌细胞）
CBP60343
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| I. General information | |
| Synonyms: | PC-3 |
| Background: | Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-alpha reductase activity. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. |
| Species: | human (Homo sapiens) |
| Tissue: | prostate |
| Disease: | Human Caucasian prostate adenocarcinoma |
| Gender: | 62 year old, male, Caucasian |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11 D5S818: 13 D7S820: 8,11 THO1: 6,7 TPOX: 8,9 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
F12K+10%FBS PC-3完全培养基，#CBP60343M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | 2n = 46, triploid, modal no. 62 |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
