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TPOR(Ligand Dependent)/BaF3

CBP73266

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I. Introduction

Cell Line Name:

TPOR(Ligand Dependent)/BaF3

Host Cell:

Ba/F3

Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Complete Culture Medium: RPMI-1640+10%FBS+2ug/ml puromycin+500nM Eltrombopag

Mycoplasma Status:

Negative

 
II. Background

Thrombopoietin receptor (TPOR) is a cytokine receptor protein present on the cell surface. The activation of TPOR by thrombopoietin (TPO) (a glycoprotein hormone) triggers an intracellular cascade of megakaryocytopoiesis for the formation of platelets. 

 
III. Representative Data

1. WB of TPOR(Ligand Dependent)/BaF3

2.Anti-proliferation assay

Figure 2. CTG Proliferation Assay of BaF3 TPOR Cells (C3), the EC50=34.1nM.


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