hMSLN/MC-38
CBPG0007
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I. Background | |||
间皮素(Mesothelin)又被称为 MSLN,它是由 MSLN 基因编码的一种细胞表面糖蛋 白。MSLN 基因编码一种前体蛋白,经蛋白水解处理生成两种蛋白产物,主要位于正常间 皮细胞表面,两种蛋白产物为巨核细胞增强因子(megakaryocyte potentiating,MPF)和间 皮素(Mesothelin,MSLN)。间皮素(MSLN)可黏附于细胞膜上,主要表达于胸腹膜与 脏器的链接面上,黏附于间皮细胞表面,在其余正常组织重表达量低。MSLN 在许多实体 瘤中呈现高表达,最常见于间皮瘤、上皮性卵巢癌和胰腺癌,但也见于肺和子宫恶性肿瘤 以及胆管癌。间皮素在卵巢癌和间皮瘤中的表达最高,较高的表达与晚期肿瘤分期和较高的转移率相关。 | |||
II. Introduction | |||
Host Cell: | MC-38 | ||
Expressed gene: | hMSLN | ||
Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
Freeze Medium: | 90% FBS+10% DMSO | ||
Culture Medium: | DMEM+10%FBS+2 μg/ml puromycin | ||
Mycoplasma Testing: | Negative | ||
Storage: | Liquid nitrogen | ||
III. Description of Host Cell Line | |||
Organism: | Mus musculus, mouse | ||
Tissue: | Colon | ||
Morphology: | Epitheloid cell | ||
Growth Properties: | Adherent | ||
IV. Representative Data | |||
Figure 1. Recombinant hMSLN/MC38 stably expressing MSLN. |
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