TLR4/NFκB-Luc/HEK293
CBP74128
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| I. Background | |
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The TLR family plays a fundamental role in pathogen recognition and activation of innate immunity. |
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| II. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: |
TLR4 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: |
MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+ 5ug/ml blasticidin+100 ug/ml hygromycin+400ug/ml G418 |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). TLR4/NF-κB Reporter - HEK293 Recombinant Cell Line (C4). |
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