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c-Kit A502_Y503dup/BaF3

CBP73296

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I. Introduction

Cell Line Name:

c-Kit A502_Y503dup/BaF3

Gene Synonyms:

c--Kit 

Host Cell:

Ba/F3

Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

 

II. Background

 
KIT A502_Y503dup (also referred to as Y503_F504insAY) indicates the insertion of 2 duplicate amino acids, alanine (A)-502 through tyrosine (Y)-503, in the Ig-like C2-type domain 5 (exon 9) of the Kit protein . A502_Y503dup results in constitutive phosphorylation of Kit and is transforming in cell culture.

 

III. Representative Data

1. WB of c-Kit A502_Y503dup/BaF3

2. Sanger of c-Kit A502_Y503dup/BaF3

3. Anti-proliferation assay

Figure 4. CTG Proliferation Assay of BaF3 C-KIT A502 _Y503dup Cells (C5).


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