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c-Kit V559D/BaF3

CBP73234

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I. Introduction

Cell Line Name:

c-Kit V559D/BaF3

Host Cell:

Ba/F3

Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

 
II. Background

KIT V559D lies within the juxtamembrane domain (exon 11) of the Kit protein (PMID: 9438854). V559D confers a gain of function on Kit, as indicated by constitutive Kit phosphorylation, and is transforming in cell culture (PMID: 9438854, PMID: 21689725).

 
III. Representative Data

1.WB of  c-Kit V559D/BaF3

 

2.Sanger of c-Kit V559D/BaF3

3. Anti-proliferation assay

Figure 2. CTG Proliferation Assay of BaF3 c-KIT V559D (C5).


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