c-Kit D816V/BaF3
CBP73206
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| I. Introduction | |
| Cell Line Name： | c-Kit D816V/BaF3 |
| Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Application: | Anti-proliferation assay and PD assay |
| Freeze Medium: | 90% FBS+10% DMSO |
| Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin |
| Mycoplasma Status: | Negative |
| II. Background | |
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Human cKit with D816V substitution, GenBank Accession No. NM_000222, a.a. 544-end with N-terminal His- tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=53 kDa. |
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| III. Representative Data | |
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1.WB of c-Kit D816V/BaF3
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2. Sanger of c-Kit D816V/BaF3
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3. Anti-proliferation assay
Figure 3. Anti-proliferation assay of three reference compounds on the c-Kit D816V/BaF3. |
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