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c-Kit Y823D/BaF3

CBP73208

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I. Introduction
Cell Line Name: c-Kit Y823D/BaF3
Host Cell: Ba/F3
Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Complete Culture Medium: RPMI-1640+10%FBS
Mycoplasma Status: Negative
 
II. Background

KIT Y823D lies within the protein kinase domain of the Kit protein (UniProt.org). Y823D confers a gain of function on Kit, as indicated by constitutive phosphorylation of Kit in cultured cells (PMID: 14695343, PMID: 20633291).

 
III. Representative Data

1.WB of  c-Kit Y823D/BaF3

2.Sanger of c-Kit Y823D/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of BaF3 c-KIT Y823D (C1).

 


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