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HTC-C3(人甲状腺癌细胞)

CBP61200

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I. General information 
Synonyms: HTC-C3
Background: established from cancer cells disseminated in the pleural fluid of a 44-year-old woman with undifferentiated giant cell carcinoma of the thyroid after chemotherapy, x-ray hyperthermia and OK432 injections in 1986; cells were described in the literature to contain parathyroid-like activity
Species: human (Homo sapiens)
Tissue: thyroid  
Disease: thyroid carcinoma
Gender: N/A 
Morphology: epithelial-like cells growing as monolayer
Growth Mode: N/A 
Doubling Time: ca. 100 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

DMEM+20%FBS

HTC-C3完全培养基,# CBP61200M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, GFAP -, neurofilament -, thyroglobulin -, vimentin -
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics and species PCR
Cytogenetics: human near-triploid karyotype with 23% polyploidy - 68(68-73)<3n>XXXXX, add(X)(p11), add(X)(p11)add(X)(q11), add(X)(q11), del(X)(p11), der(6)t(X;6)(q23;q25), inc.
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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