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CAL-62(人甲状腺癌细胞)

CBP60709

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I. General information 
Synonyms: CAL-62
Background: established from the thyroid gland (right lobe) of a 70-year-old woman with thyroid anaplastic carcinoma in 1988; described as being tumorigenic in heterotransplanted nude mice
Species: human (Homo sapiens)
Tissue: thyroid
Disease: thyroid anaplastic carcinoma
Gender: N/A 
Morphology: epithelial-like cells growing in monolayers; i
Growth Mode: N/A 
Doubling Time: ca. 24 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

DMEM+10%FBS

CAL-62完全培养基,# CBP60709M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin +, endothel -, EpCAM +, GFAP -, neurofilament +, thyroglobulin -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: N/A 
Cytogenetics: human, flat-moded hypertriploid karyotype with 8% polyploidy - 60-76<3n>XX, -X, +3, +3, -4, +6, +7, +9, -11, -14, -18, +19, +20, +20, +2-6mar - del(1)(p34), der(1)t(1;17)(p34;q21), der(3)add(3)(p11)?dup(3)(q21q22)x2-4, del(6)(q15)x2, i(8q), der(?12)t(1;12)(q11-12;q14), add(17)(q21), add(21)(q22) - sideline with del(X)(q24), der(7;8)(q10;q10) - resembles published karyotype
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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