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BHT-101(人甲状腺癌细胞)

CBP60706

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I. General information 
Synonyms: BHT-101
Background: established from the lymph node metastasis of a 63-year-old woman with anaplastic papillary thyroid carinoma; cells were described in the literature to not produce hormones, but to be partly positive for thyroglobulin- and thyroxine (T4)
Species: human (Homo sapiens)
Tissue: thyroid
Disease: thyroid carcinoma
Gender: N/A 
Morphology: epithelial-like cells growing in monolayers; 
Growth Mode: N/A 
Doubling Time: about 2-3 days
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

DMEM+20%FBS

BHT-101完全培养基,# CBP60706M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP +, neurofilament -, thyroglobulin -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, G6PD, LDH, NP
Cytogenetics: apparently human hypertetraploid karyotype
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
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