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DMS53(人小细胞肺癌细胞)

CBP60172

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I. General information 
Synonyms: DMS53
Background: The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.
Species: Homo sapiens, human
Tissue: lung
Disease: carcinoma; small cell lung cancer
Gender: male,54 years,Caucasian
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 12,13
D5S818: 10,11
D7S820: 8,11
THO1: 8,9.3
TPOX: 12
vWA: 15,17
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+10%FBS

DMS53完全培养基,# CBP60172M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: Leu 7; My23
Receptor Expression: bombesin, expressed.epidermal growth factor (EGF), expressed
transforming growth factor beta (TGF beta), expressed,acetylcholine, expressed
Genes Expressed: adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; human chorionic gonadotropin (hCG); glucagon; growth hormone; 17 beta estradiol; thyroid releasing hormone; oxytocin - neurophysin (OT-NP);  parathormone;,somatostatin-like immunoreactivity (SRIF)
Cellular Products: adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; calcitonin; human chorionic gonadotropin (hCG); glucagon; growth hormone; 17 beta estradiol; thyroid releasing hormone; oxytocin - neurophysin (OT-NP); parathormone;
somatostatin-like immunoreactivity (SRIF)
Tumor Formation: Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells.
Comments: The patient had not received prior therapy. The cells express HLA class I and class II antigens.
Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin derived products.
For more information, please contact Cobioer (4008-750-250).

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