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SVCT(人SV40转化乳腺上皮细胞)

CBP61166

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I. General information 
Synonyms: SVCT
Background: SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.
Species: human (Homo sapiens)
Tissue: breast
Disease: Human breast epithelium, SV40 transformed
Gender: N/A
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10µg/ml Insulin + 10%FBS
Cobioer’s Cell Line Authentication Service
Culture Medium:

DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10µg/ml Insulin + 10%FBS

SVCT完全培养基,# CBP61166M

We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Not specified
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Comments: For more information, please contact Cobioer (4008-750-250).

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