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CAL-120(人乳腺癌细胞)

CBP60358

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I. General information 
Synonyms: CAL-120
Background: established from the pleural effusion of a 43-year-old woman with metastatic breast adenocarcinoma in 1991
Species: human (Homo sapiens)
Tissue: breast 
Disease: breast adenocarcinoma
Gender: N/A 
Morphology: heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers
Growth Mode: N/A 
Doubling Time: ca. 50-60 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

DMEM+10%FBS

CAL-120完全培养基,# CBP60358M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics
Cytogenetics: human hypertetraploid karyotype with 20% polyploidy 98(88-98)<4n>XXX/XXXX, -1, -2, -2, -4, +5, +5, +6, -9, -9, +10, +11, -12, -12, -13, -13, -14, -15, -16, -16, -20, -21, -22, +16-18mar, + 1-2 dmin, add(X)(p2?2), add(1)(q22), del(3)(q22), add(3)(p1?4), del(4)(p15.2), add(6)(q11)x2, del(7)(q32), add(8)(p12), add(8)(q24), der(8;13)(q10;q10), ins(11;?)(q13;?), add(12)(p1?), add(13)(p11)x1-2, add(14)(p11), der(15;?21)(p1?1;?q12)x3 - sideline with i(1)(p10)add(1)(p3?2), i(1q), i(3p), der(16)t(5;16)(q13;q24)
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -
Comments: For more information, please contact Cobioer (4008-750-250).
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