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hTERT-HPNE(人胰腺导管细胞)

CBP60857

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I. General information 
Synonyms: hTERT-HPNE
Background: The hTERT-HPNE cell line was developed from human pancreatic duct by transduction with a retroviral expression vector (pBABEpuro) containing the hTERT gene. The infected cells became positive for telomerase, failed to senesce, and were still proliferating after more than 150 doublings Ref.
Species: Homo sapiens, human
Tissue: Pancreas, duct
Disease: Normal
Gender: Male, 52 years
Morphology: Epithelial-like
Growth Mode: adherent
DNA Profile: D5S818: 11
D13S317: 12,13
D7S820: 9,10
D16S539: 12,13
vWA: 17
Amelogenin: X,Y
TPOX: 8,11
CSF1PO: 12
TH01: 8,9
Cobioer’s Cell Line Authentication Service
Culture Medium:

DMEM+25%M3 medium+5%FBS+10ng/ml EGF

hTERT-HPNE完全培养基,# CBP60857M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Comments: Exposing hTERT-HPNE cells to sodium butyrate and 5-aza-2'-deoxycytidine lead to the formation of pancreatic ductal cells marked by the expression of p-glycoprotein (multidrug resistance (MDR-1)), carbonic anhydrase II, and the cytokeratins 7, 8, and 19.
hTERT-HPNE cells were found to have properties of the intermediary cells formed during acinar-to-ductal metaplasia, which included their undifferentiated phenotype, expression of Nestin and, evidence of active Notch signaling Ref.
For more information, please contact Cobioer (4008-750-250).
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