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HPAF-II(人胰腺腺癌细胞)

CBP60836

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I. General information 
Synonyms: HPAF-II
Background: HPAF-II is a human pancreatic adenocarcinoma cell line derived from peritoneal ascitic fluid of a 44 year old Caucasian male with primary pancreatic adenocarcinoma and metastases to the liver, diaphragm and lymph nodes.­
Species: Homo sapiens, human
Tissue: pancreas
Disease: adenocarcinoma
Gender: male,  44 years,  Caucasian
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 11,13
D5S818: 11,13
D7S820: 10,13
F13A01: 5,17
F13B: 8,10
FESFPS: 11,12
LPL: 10
THO1: 9
TPOX: 8
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium:

MEM+10%FBS+1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)

HPAF-II完全培养基,# CBP60836M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: Blood Type A; Rh-; HLA A1, A10 (W34), B8, W22, Cw3
Genes Expressed: mucin
Cellular Products: mucin
Tumor Formation: Yes, the cells form well differentiated adenocarcinomas in athymic mice which resemble the original tumor
Comments: HPAF II is a spontaneous variant with unlimited replicative capability which proliferated from a static culture of HPAF-I cells. The cells express Muc 1 and Muc 4 mucin genes, and secrete high levels of Muc 1 mucin. They are pleomorphic and probably undergo some spontaneous differentiation in culture
For more information, please contact Cobioer (4008-750-250).

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