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Panc 10.05(人胰腺腺癌细胞)

CBP60846

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I. General information 
Synonyms: Panc 10.05
Background: Panc 10.05 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.
The Panc 10.05 cell line was derived from the same patient as the PL45 cell line (ATCC CRL-2558).
Species: Homo sapiens, human
Tissue: pancreas
Disease: adenocarcinoma
Gender: male, adult, Caucasian
Morphology: epithelial
Growth Mode: adherent
Doubling Time: 19.2 hrs
DNA Profile: D5S818: 13
D13S317: 12
D7S820: 8,9
D16S539: 9,12
vWA: 16
THO1: 6,9.3
Amelogenin: X
TPOX: 11
CSF1PO: 12
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+15%FBS+10ug/ml insulin

Panc 10.05完全培养基,# CBP60846M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: MHC class I +; MHC class II -
Receptor Expression:  
Oncogene: K-ras + Ref
Genes Expressed: cytokeratins 7 and 18
Ref
Cellular Products: cytokeratins 7 and 18
Ref
Tumor Formation: Yes, forms tumors in nude or SCID mice
Comments: Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine.
The cells have a reported plating efficiency of 40%.
For more information, please contact Cobioer (4008-750-250).

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