NTERA-2 cl.D1（人睾丸癌细胞）
CBP60680
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| I. General information | |
| Synonyms: | NTERA-2 cl.D1 |
| Background: | The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line (see ATCC HTB-106). The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line. |
| Species: | Homo sapiens, human |
| Tissue: | testis; derived from metastatic site: lung |
| Disease: | malignant pluripotent embryonal carcinoma |
| Gender: | Caucasian, male, 22 years |
| Morphology: | epithelial-like,differentiation changes phenotype |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 13 D16S539: 11,12,13 D5S818: 9,12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 18,19 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
DMEM +10%FBS NTERA-2 cl.D1完全培养基，# CBP60680M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | This clone differentiates along neuroectodermal lineages after exposure to retinoic acid (RA) or hexamethylene bisacetamide (HMBA).The RA induced differentiation is characterized by glycolipid changes, appearance of neurons, and induction of homeobox (HOX) gene clusters. The cells exhibit high expression of N-myc oncogene activity.To induce differentiation, the cells should be trypsinized and seeded at a density 1 X 10 exp6 cells per 75 sq. cm. in medium containing 0.01 mM trans-retinoic acid. Stock solutions of trans-retinoic acid (10 mM, dissolved in DMSO) should be stored frozen (preferably under a nitrogen atmosphere). For more information, please contact Cobioer (4008-750-250). |
