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WILL-2(人B细胞淋巴瘤)

CBP60624

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I. General information 
Synonyms: WILL-2
Background: established in 2008 post-mortem from the ascites of a 63-year-old Japanese woman with diffuse large B-cell lymphoma after relapse
Species: human (Homo sapiens)
Tissue: N/A 
Disease: B cell lymphoma
Gender: N/A 
Morphology: small and mostly single round cells growing in suspension; 
Growth Mode: N/A 
Doubling Time: ca. 36-48 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+20%FBS

WILL-2完全培养基,# CBP60624M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD38 +, CD138 +, HLA-DR +; 
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique STR DNA profile
Species: N/A 
Cytogenetics: human near-diploid karyotype; 46(43-47)<2n>XX, del(X)(q23), dup(1)(q21q32), ins(1;X)(q21;q27q23), t(4;17)(p15;q12). t(5;9)(q14;p21), t(8;22)(q24;q11), t(14;18)(q32;q21); carries t(8;22) rearrangement effecting IGL-MYC, and t(14;18) rearrangements effecting IGH-BCL2; resembles published karyotype
Molec. Genetics: expression of fusion gene IGH-BCL2 confirmed by RT-PCR
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Comments: For more information, please contact Cobioer (4008-750-250).
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