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BC-3(人胸膜积液B淋巴细胞)

CBP60818

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I. General information 
Synonyms: BC-3
Background: The cells were established from pleural effusion from a human immunodeficiency virus (HIV) negative patient diagnosed with primary effusion (body cavity based) lymphoma (PEL).
BC-3 is a primary effusion lymphoma cell line originated in 1995 by Leandros Arvanitakis and Ethel Cesarman.
Species: Homo sapiens, human
Tissue: Pleura, pleural effusion
Disease: lymphoma
Gender: male, 85 years, Caucasian
Morphology: lymphoblast
Growth Mode: suspension
DNA Profile: Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 14,18
Cobioer’s Cell Line Authentication Service
Culture Medium: RPMI-1640+20%FBS
We strongly suggest to purchase the complete medium from Cobioer.
Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: CD30 +; CD38 +; CD45 +; CD 54 +; CD71 +; HLA-DR +; EMA + (epithelial membrane antigen); CD2 -; CD3 -; CD4 -; CD5 -, CD8 -; CD19 -; CD20 -; CD21 -; CD22 -
Comments:  The BC-3 cell line contains the viral genome for Kaposi's sarcoma-associated herpesvirus (KSHV, provisionally designated HHV-8).
KSHV is a recently identified and partially uncharacterized virus.
BC-3 cells are negative for both HIV and Epstein-Barr virus (EBV).
They are also reported to be negative for herpes simplex virus 1 & 2 (HSV-1 and HSV-2) and cytomegalovirus (CMV).
The BC-3 cell line allows in vitro culture of KSHV viral genomes in the absence of EBV.
BC-3 has an intact KSHV genome of about 170 kb both in the cell line and in the viral isolate.
The cells do not express B-cell lineage restricted antigens or kappa or lambda immunoglobulin light chains or T-cell lineage-restricted antigens.
The cells do express activation antigens.
For more information, please contact Cobioer (4008-750-250).

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