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OCI-LY19(人B细胞淋巴瘤)

CBP60621

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I. General information 
Synonyms: OCI-LY19
Background: established from the bone marrow of a 27-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL) (diffuse large cell lymphoma, DLCL, stage 4B, at relapse) in 1987
Species: human (Homo sapiens)
Tissue: N/A 
Disease: B cell lymphoma
Gender: N/A 
Morphology: single round cells growing in suspension;
Growth Mode: N/A 
Doubling Time: ca. 40 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

80% MEM α (GIBCO 12561-056 ) + 20% FBS

OCI-LY19完全培养基,# CBP60621M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD37 -, CD38 +, cyCD79a +, CD80 +, CD138 +, HLA-DR +;  
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics
Cytogenetics: human hyperdiploid karyotype with 4% polyploidy - 48(46-52)<2n>X, -X, +6, +6, +8, t(4;8)(q3?2;q?24), del(6)(q15)x2, r(8)(??), t(14;18)(q32;q21), add(18)(q23) - carries t(14;18) effecting IGH-BCL2 juxtaposition
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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