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MCF 10A(人正常乳腺上皮细胞)

CBP60419

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I. General information 
Synonyms: MCF 10A
Background: MCF 10A was derived from adherent cells in the population.
Species: Homo sapiens, human
Tissue: mammary gland; breast
Disease: fibrocystic disease
Gender: female, female, Caucasian
Morphology: epithelial
Growth Mode: adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 15,17
Cobioer’s Cell Line Authentication Service
Isoenzymes: AK-1, 1
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ES-D, 1
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G6PD, B
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GLO-I, 1-2
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PGM1, 1-2
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PGM3, 1
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Culture Medium:

MEGM+100 ng/ml cholera toxin

MCF 10A完全培养基,# CBP60419M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: N/A
Receptor Expression: N/A
Oncogene: N/A
Genes Expressed: N/A
Cellular Products: N/A
Tumor Formation: No, in immunosuppressed mice
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Yes, in semisolid medium
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Comments: The MCF 10A cell line is a non-tumorigenic epithelial cell line.
The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen.
They exhibit three dimensional growth in collagen, and form domes in confluent cultures.
Thus far, the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF).
By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells.
They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies.
The calcium content of the medium exerts a strong effect on the morphology of the cells.
For more information, please contact Cobioer (4008-750-250).
药靶模型联系方式: 华东销售经理:18240630236 全国销售经理:18066071954
诊断标准品联系方式: 华东销售经理:15000320447 华北销售经理:18131625521 华南销售经理:13484295986 华中&西南销售经理:13871580511 全国销售经理:13484295986

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