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EFM-192C(人乳腺癌细胞)

CBP60366

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I. General information 
Synonyms: EFM-192C
Background: established from the pleural effusion of the left side (14 days after EFM-192A) of a 46-year-old Caucasian woman with breast adenocarcinoma; sister cell lines EFM-192A and -192B show differences in molecular aspects; erbB-2 gene is amplified and overexpressed
Species: human (Homo sapiens)
Tissue: breast  
Disease: breast carcinoma
Gender: N/A 
Morphology: epitheloid adherent cells growing as monolayers
Growth Mode: N/A 
Doubling Time: ca. 4 days
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+20%FBS

EFM-192C完全培养基,# CBP60366M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin -
Fingerprint: same DNA profile as EFM-192A/EFM-192B using multiplex PCR of minisatellite markers
Species: confirmed as human with IEF of AST, MDH, NP
Cytogenetics: highly rearranged human hypohexaploid karyotype with 10% polyploidy - 122-138<6n>XXXXX/XXXXXX - related to EFM-192A and EFM-192 B
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
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