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BRAF V600R/BaF3

CBP73385

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I. Introduction
Cell Line Name: BRAF V600R/BaF3
Host Cell: Ba/F3
Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application: Anti-proliferation assay and PD assay
Freeze Medium: 90% FBS+10% DMSO
Complete Culture Medium: RPMI-1640+10%FBS+2ug/ml puromycin
Mycoplasma Status: Negative
 
II. Background
BRAF V600R (previously reported as V599) lies within the activation segment of the kinase domain of the Braf protein. V600R confers a gain of function to the Braf protein as demonstrated by increased Braf kinase activity, downstream signaling, and the ability to transform cells in culture.
 
III. Representative Data

1. WB of BRAF V600R/BaF3

2.Sanger of BRAF V600R/BaF3

Figure 2. Sanger of BRAF V600R/BaF3

3. Anti-proliferation assay

Figure 3. CTG Proliferation Assay of B-RAF V600R BaF3 Cells (C14).

 

 

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