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KRAS G12V/BaF3

CBP73230

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I. Introduction

Cell Line Name:

KRAS G12V/BaF3

Host Cell:

Ba/F3

Stability: 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Freeze Medium: 90% FBS+10% DMSO
Complete Culture Medium: RPMI-1640+10%FBS+1ug/ml puromycin
Mycoplasma Status: Negative
 
II. Background

Human KRAS also known as C-K-RAS, CFC2, and K-RAS2A, GenBank Accession No. NM_033360, a.a. 2-185, with G12V mutation and N-terminal His-tag, and expressed in a Baculovirus infected Sf9 cell expression system. MW=22 kDa.

 
III. Representative Data

1..WB of KRAS G12V/BaF3

2.Anti-proliferation assay

Figure 3. Anti-proliferation assay of two reference compounds on the KRAS G12V/BaF3 Stable Cell Line.

 

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