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CTLA4/IL2 Promoter Reporter/Jurkat

CBP74084

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I. Background

CTLA4 is a member of the immunoglobulin superfamily. It is expressed by activated T cells and transmits an inhibitory signal to T cells. CTLA4 is homologous to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells.

 
II. Description
Recombinant Jurkat T cell expressing firefly luciferase gene under the control of IL-2 promoter with constitutive expression of human CTLA4.
 
III. Introduction
Host Cell: Jurkat
Expressed gene: IL2 Promoter
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:

RPMI-1640+10%FBS+1ug/ml puromycin+10ug/ml blasticidin

Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
IV. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Peripheral blood
Disease: Childhood T acute lymphoblastic leukemia
Morphology: Lymphoblast
Growth Properties: Suspension
 
Ⅴ. Representative Data

Figure 1. Recombinant Jurkat stably expressing human CTLA4.

 

Figure 2. Dose Response of lpilimumab in CTLA4/IL2 Promoter Reporter-Jurkat Cells (C39) with TCR Activator Cell, the EC50=2.83μg/ml .

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