TCR Activator/Raji
CBP74015
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| I. Background | |
| T cells are critical for protective immune responses to pathogens and tumors. The T-cell receptor (TCR)–CD3 complex is composed of a diverse αβ TCR heterodimer noncovalently associated with the invariant CD3 dimers CD3ϵγ, CD3ϵδ, and CD3ζζ. The TCR mediates recognition of antigenic peptides bound to MHC molecules (pMHC), whereas the CD3 molecules transduce activation signals to the T cell. | |
| II. Description | |
| Recombinant RAJI cell constitutively expressing a membrane bound, engineered T cell receptor (TCR) activator. | |
| III. Introduction | |
| Host Cell: | RAJI |
| Expressed gene: | TCR activator |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+400ug/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Homo sapiens,human |
| Tissue: | Lymphoblast |
| Disease: | Burkitt's lymphoma |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| Ⅴ. Representative Data | |
Figure 1. Jurkat NFAT Luciferase Reporter Activity with NFAT-Luc/Jurkat.
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Figure 2. Response of IL-2-Luciferase Reporter Jurkat Cell Line (C29) to Raji TCR Activator Cell Line.
Figure 3. Dose Response of Recombinant hCTLA4-Fc in IL2 Promoter Reporter-Jurkat Cells with TCR Activitor Raji Cells.
