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hGM-CSF Effector Reporter Cell

CBP74149

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I. Background

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine stimulating the production of granulocytes and monocytes from bone marrow precursors. GM-CSF had pro-inflammatory functions and is a therapeutic target in autoimmune disease. GM-CSF signals through JAK2/STAT5 and stimulates the expression of STAT5 target genes.

 
II.. Introduction
Expressed gene: hGM-CSF
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium: RPMI-1640+10% FBS+100ug/ml hygromycin+2 ng/ml GM-CSF
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Representative Data


Figure 1. Dose Response of Recombinant Human GM-CSF in hGM-CSF Effector Reporter Cells (Clone 10).

Figure 2. Inhibition of hGM-CSF-induced Reporter Activity by hGM-CSF Neutralizing Antibody in hGM-CSF Effector Reporter Cells (Clone 10).

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