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TLR3/NFκB-Luc/HEK293

CBP74057

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I. Background

TLR3 (Toll Like Receptor 3) is a Protein Coding gene. Diseases associated with TLR3 include Encephalopathy, Acute, Infection-Induced 2 and Human Immunodeficiency Virus Type 1. Among its related pathways are Activated TLR4 signalling and Toll-like Receptor Signaling Pathway. Gene Ontology (GO) annotations related to this gene include identical protein binding and transmembrane signaling receptor activity.

 
II. Introduction
Host Cell: HEK293
Expressed gene: TLR3、NFκB-Luc
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:
MEM+10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+100ug/ml hygromycin
Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Description of Host Cell Line
Organism: Human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid cell
Growth Properties: Adherent
 
IV. Representative Data


Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). TLR3/NF-κB Reporter-HEK293 cells were stimulated by Poly(I:C), the S/B was 57-fold.

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