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TLR4/NFκB-Luc/HEK293

CBP74128

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I. Background

The TLR family plays a fundamental role in pathogen recognition and activation of innate immunity.

 
II. Introduction
Host Cell: HEK293
Expressed gene:

TLR4

Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:

MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1ug/ml puromycin+ 5ug/ml blasticidin+100 ug/ml hygromycin+400ug/ml G418

Mycoplasma Testing: Negative
Storage: Liquid nitrogen
Application(s): Functional(Report Gene) Assay
 
III. Description of Host Cell Line
Organism: Human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epitheloid
Growth Properties: Adherent
 
IV. Representative Data

Figure 1.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). TLR4/NF-κB Reporter - HEK293 Recombinant Cell Line (C4).

 

 

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