NCI-H292（人粘液表皮样癌肺）
CBP60187
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| I. General information | |
| Synonyms: | NCI-H292 |
| Background: | This line was derived from a lymph node metastasis of a pulmonary mucoepidermoid carcinoma. The cells were isolated in a chemically defined medium (HITES) and later adapted to growth in media supplemented with serum. |
| Species: | Homo sapiens, human |
| Tissue: | lung |
| Disease: | mucoepidermoid pulmonary carcinoma |
| Gender: | female, 32 years, Black |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 48 hrs |
| DNA Profile: | Amelogenin: X CSF1PO: 10 D13S317: 11,12 D16S539: 9,13 D5S818: 13 D7S820: 10 THO1: 8 TPOX: 8,11 vWA: 16,17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H292完全培养基，# CBP60187M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | keratin; vimentin,The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. TANK cells show reactivity with human alloantisera specific for the HLA A1, A3, A10, A19, B12, B17, B22 and B40 cross-reactive groups. |
| Cellular Products: | keratin; vimentin |
| Tumor Formation: | Yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features Hepatitis B virus |
| Comments: | The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. The cells support the growth of hepatitis B virus and are negative for L-DOPA decarboxylaseThe cells stain positive for keratin and vimentin and are mucicarmine positive but are negative for neurofilament triplet protein. For more information, please contact Cobioer (4008-750-250). |
