NCI-H441（人肺癌腺癌细胞）
CBP60137
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| I. General information | |
| Synonyms: | NCI-H441 |
| Background: | The NCI-H441 cell line was derived by A.F. Gazdar, M. Brower and D. Carney and associates in 1982 from the pericardial fluid of a patient with papillary adenocarcinoma of the lung. |
| Species: | Homo sapiens, human |
| Tissue: | lung |
| Disease: | papillary adenocarcinoma |
| Gender: | male |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| Doubling Time: | 58 hrs in medium with serum; 99 to 138 hrs in serum-free medium |
| DNA Profile: | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9 D16S539: 9,13 D5S818: 11,12 D7S820: 10 THO1: 9.3 TPOX: 8,10 vWA: 17 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H441完全培养基，# CBP60137M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Comments: | The cell line expresses mRNA and protein of the major surfactant apoprotein (SP-A). Electron microscopy shows multilamellar bodies and cytoplasmic structures resembling clara cell granules.The cells can be cloned in soft agar with or without serum. For more information, please contact Cobioer (4008-750-250). |
