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NCI-H322(白种人的细支气管肺泡癌)

CBP60134

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I. General information 
Synonyms: NCI-H322
Background: NCI-H322 was derived in 1981 from a primary bronchioalveolar carcinoma of the lung from a 52 year old male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma cell line demonstrated the presence of cytoplasmic structures characteristic of Clara cells. NCI-H322 do not express UDP-glucuronosyltransferase, but are reported to express glutathione-S-transferase and phenol sulfotransferase. Expression of protein and RNA of SP-A, the major surfactant-associated protein was detected. The cells produce tumours in athymic mice.
Species: human (Homo sapiens)
Tissue: Lung (cervical node metastasis)
Disease: Human Caucasian bronchioalveolar carcinoma
Gender: a 52 year old male
Morphology: N/A
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X,Y
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 11
D7S820: 11
THO1: 7
TPOX: 8
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+10%FBS

NCI-H322完全培养基,# CBP60134M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Not specified
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Comments: For more information, please contact Cobioer (4008-750-250).
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