LC-2/ad（人肺癌腺癌细胞）
CBP60104
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| I. General information | |
| Synonyms: | LC-2/ad |
| Background: | Established from the pleural effusion of a pulmonary adenocarcinoma in a 51 year-old Japanese female. LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes. Immunocytochemically the cells are positive for CEA and cytokeratins, including cytokeratin No.18. LC-2/ad produces at least two functionally active trypsin inhibitors together with several proteinases in vitro. |
| Species: | human (Homo sapiens) |
| Tissue: | Lung |
| Disease: | Human lung adenocarcinoma |
| Gender: | 51 year-old Japanese female |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Amelogenin: X, X CSF1PO: 10,11 D13S317: 10,12 D16S539: 9 D5S818: 10 D7S820: 11 THO1: 7 TPOX: 11 vWA: 16,19 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640: F12 (1:1) + 2mM Glutamine+10%FBS LC-2/ad完全培养基，# CBP60104M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Modal No 53-56 |
| Subculture Routine: | This cell line is relatively slow growing taking about 5 days to reach 70 -80% confluency. Split cultures 1:2-1:4 ie seeding at 2-5 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/ EDTA ; 5%CO2; 37°C |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
