HCC-15（人肺癌细胞（非小细胞））
CBP60096
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| I. General information | |
| Synonyms: | HCC-15 |
| Background: | established from the tumor of a 47-year-old man with non-small cell lung carcinoma (subtype squamous carcinoma); matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-15BL) |
| Species: | human (Homo sapiens) |
| Tissue: | lung |
| Disease: | non-small cell lung carcinoma |
| Gender: | N/A |
| Morphology: | adherent epitheloid cells growing as monolayer |
| Growth Mode: | N/A |
| Doubling Time: | ca. 30-40 hours |
| DNA Profile: | N/A Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS HCC-15完全培养基，# CBP60096M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin + |
| Fingerprint: | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Species: | confirmed as human by cytogenetics |
| Cytogenetics: | human hypodiploid karyotype with 16% polyploidy - 37(34-38)<2n>X, -X, -4, -13, -14, -17, -18, -19, -20, -22, +1-2mar, del(3)(q13), der(5;19)(q10;q10)add(19)(q13), add(9)(p2?), del(10)(p12-13), add(12)(q24.3), del(20)(?p12), add(22)(q1?) - sideline with add(8)(p12), del(7)(q21) |
| Virus | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
