NCI-H1299（人非小细胞肺癌细胞）
CBP60053
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| I. General information | |
| Synonyms: | NCI-H1299 |
| Background: | The cell line was established from a lymph node metastasis of the lung from a patient who had received prior radiation therapy. |
| Species: | Homo sapiens, human |
| Tissue: | lung; derived from metastatic site: lymph node |
| Disease: | carcinoma; non-small cell lung cancer |
| Gender: | male, 43 years adult, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | adherent |
| DNA Profile: | Amelogenin:X CSF1PO:12 D13S317:12 D16S539:12,13 D5S818:11 D7S820:10 THO1:6,9.3 TPOX:8 vWA: 16,17,18 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS NCI-H1299完全培养基，# CBP60053M |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | These cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein. neuromedin B,The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells produce neuromedin B. |
| Cellular Products: | neuromedin B |
| Comments: | The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. They reported to be able to synthesize the peptide neuromedin B (NMB) at 0.1 pmol/mg protein, but not the gastrin releasing peptide (GRP). For more information, please contact Cobioer (4008-750-250). |
