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LCLC-97TM1(人大细胞肺癌)

CBP60048

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I. General information 
Synonyms: LCLC-97TM1
Background: cell line represents the xenotransplant of a primary tumor which was obtained from a 44-year-old Caucasian man with large cell lung carcinoma who did not have any prior therapy
Species: human (Homo sapiens)
Tissue: lung
Disease: large cell lung carcinoma
Gender: N/A 
Morphology: adherent epitheloid cells, densely packed in islets, growing as monolayers; image; image
Growth Mode: N/A 
Doubling Time: >50 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+10%FBS

LCLC-97TM1完全培养基,# CBP60048M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+)
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, LDH, MDH
Cytogenetics: human hyperdiploid/hypotriploid karyotype - 58(50-60)<3n>X, -X/Y, -X/Y, +1, +1, +5, +5, -7, -10, -11, +12, -13, -15, -15, -15, -17, -18, -18, -19, -20, -21, -21, -22, der(1;7)(q10;q10)x2, der(1;15)(p10;q10)x2, der(3;6)(q10;q10), der(5;18)(p10;p10), der(5;18)(q10;q10), der(6;10)(p10;q10), i(7p)x2, i(8p), i(8q)x2, der(11)t(1;11)(q11;q24), der(14)t(7;14)(q12;q32), der(14)t(7;14)(q12;q32)add(14)(p11), der(14)t(7;14)(q12;q32)t(14;21)(p10;q10) - carries an unprecedented cluster of whole-arm translocations - resembles published karyotype
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).
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