首页 /科研细胞 /科研细胞 /科研细胞 /LS180(人结肠癌肿瘤细胞)

LS180(人结肠癌肿瘤细胞)

CBP60034

询 价
索取STR
产品描述
产品数据库
I. General information
Synonyms: LS180
Background: The cell line was established from a Duke's type B adenocarcinoma of the colon. The tissue was minced and cultured without transfer for 10 months.
Species: Homo sapiens, human
Tissue: colon
Disease: Dukes' type B,colorectal adenocarcinoma
Gender: female, 58 years,Caucasian
Morphology: epithelial
Growth Mode: adherent
DNA Profile: Amelogenin: X
CSF1PO: 10, 13
D13S317: 10
D16S539: 11, 13
D5S818: 10, 15
D7S820: 11, 9. 3
THO1: 6, 7
TPOX: 8, 9
vWA: 15, 18
Cobioer’s Cell Line Authentication Service
Culture Medium:

MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)

LS180完全培养基,# CBP60034M

We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: serologically defined colon cancer antigen 3; Homo sapiens, expressed
HLA A2, B13, B50; Blood type O
Oncogene: myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -
Genes Expressed: carcinoembryonic antigen (CEA), interleukin 10 (IL-10), interleukin 6 (IL-6), mucin,The production of CEA in the ATCC seed stock was 1944 ng per 10(6) cells in 10 days.
Cellular Products: carcinoembryonic antigen (CEA).interleukin 10 (IL-10),interleukin 6 (IL-6),mucin
Tumor Formation: Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Comments: LS 174T is a variant of LS 180 (ATCC CL-187) that has been maintained by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA).Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles. Ref
They are negative for p53 antigen expression, but positive for mRNA expression.LS 174T cells stain positively for cytokeratins. The line is positive for expression of c-myc, N-myc, H-ras, N-ras, Myb, and fos oncogenes. Ref,K-ras and sis oncogene expression were not detected.
For more information, please contact Cobioer (4008-750-250).
药靶模型联系方式: 华东销售经理:18240630236 全国销售经理:18066071954
诊断标准品联系方式: 华东销售经理:15000320447 华北销售经理:18131625521 华南销售经理:13484295986 华中&西南销售经理:13871580511 全国销售经理:13484295986

扫二维码

立即提交