Colo-205（人结肠癌肿瘤细胞）
CBP60026
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| I. General information | |
| Synonyms: | Colo-205 |
| Background: | This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. |
| Species: | Homo sapiens, human |
| Tissue: | colon; derived from metastatic site: ascites |
| Disease: | Dukes' type D, colorectal adenocarcinoma |
| Gender: | male, 70 years, Caucasian |
| Morphology: | epithelial |
| Growth Mode: | mixed, adherent and suspension |
| DNA Profile: | Amelogenin: X CSF1PO: 11,12 D13S317: 10,12 D16S539: 12,13 D5S818: 10,13 D7S820: 9,10 THO1: 8,9 TPOX: 11 vWA: 15 Cobioer’s Cell Line Authentication Service |
| Culture Medium: |
RPMI-1640+10%FBS We strongly suggest to purchase the complete medium from Cobioer.
|
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Genes Expressed: | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10),The cells are positive for keratin by immunoperoxidase staining. |
| Cellular Products: | carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10) |
| Tumor Formation: | Yes, in nude mice,(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments: | The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224). The cells are CSAp negative (CSAp-). The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. For more information, please contact Cobioer (4008-750-250). |
