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LS1034(人结肠癌细胞)

CBP60013

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I. General information 
Synonyms: LS1034
Background: LS1034 is a colorectal carcinoma cell line isolated in 1989 from a primary tumor biopsy from a 54-year old Caucasian male patient diagnosed with Dukes' Type C, moderately to poorly differentiated cecal carcinoma.
Species: Homo sapiens, human
Tissue: cecum
Disease: Dukes' type C, colorectal carcinoma
Gender: male, 54 years  adult,Caucasian
Morphology: epithelial  
Growth Mode: adherent, adherent
Doubling Time: 33hrs
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,11
D5S818: 12,13
D7S820: 8,12
THO1: 7
TPOX: 9,11
vWA: 17
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+10%FBS

LS1034完全培养基,# CBP60013M

We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Antigen Expression: carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive
Oncogene: p53 + (mutated, Gly --> Ser mutation at position 245), APC (mutated, deletion, GAAAAGATT --> GATT at codon 1309)
Tumor Formation: Yes, forms tumors in nude mice
Comments: More than 90% of LS1034 cells express surface CEA.The cells express the major histocompatibility (MHC) class I antigens and beta 2 microglobulin, but class II antigens, (HLA-DR, DQ, and DP) were not detected. No measurable amount of latent transforming growth factor beta-1 (TGF beta-1) is secreted.
Picomolar concentrations of TGF beta-1, TGF beta-2, and TGF beta-3 inhibit the proliferation of LS1034 cells. LS1034 cells are multidrug resistant (MDR), and can proliferate in serum free medium.The patient did not exhibit familial adenomatous polyposis (FAP) and normal tissue did not have the deletion in the APC gene. The colony forming efficiency was 3% in methylcellulose medium containing 5% fetal bovine serum, and the clonogenicity is increased 600% in serum free methylcellulose as compared to semisolid medium containing serum.  A culture submitted to the ATCC in September 1994 was found to be contaminated with mycoplasma, and was cured by a 21 day treatment with BM Cycline.
For more information, please contact Cobioer (4008-750-250).
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