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OCI-M2(人急性粒细胞白血病)

CBP61206

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I. General information 
Synonyms: OCI-M2
Background: established in 1984 from the leukemic cells of a 56-year-old patient with erythroleukemia (AML-M6), representing the end stage of a previously identified myelodysplastic syndrome; described in the literature to differentiate along the erythroid or megakaryocytic/monocytic lineage after stimulation with erythroid inducers or phorbol esters, respectively; described in the literature to constitutively and inducibly secrete platelet-derived growth factor (PDGF)-like proteins; contamination with Mycobacterium was eliminated with Kanamycin
Species: human (Homo sapiens)
Tissue: N/A 
Disease: acute myelocytic leukemia
Gender: N/A 
Morphology: round cells growing singly or in clusters in suspension, occasionally a few cells become adherent;
Growth Mode: N/A 
Doubling Time: ca. 36 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

IMDM+20%FBS

OCI-M2完全培养基,# CBP61206M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, CD71 +, CD235a +; 
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics
Cytogenetics: human hyperdiploid karyotype with 4% polyploidy; 51(46-51)<2n>XX, +6, +8, +20, -21, +3mar, der(X)t(X;8)(q23;q23), add(9)(p23)del(9)(p12p21), t(10;12)(p12;p12), del(17)(q11q21.1); no reference karyotype available
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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