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EFE-184(人子宫内膜癌细胞)

CBP61196

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I. General information 
Synonyms: EFE-184
Background: established from the ascitic fluid of a 69-year-old Caucasian woman with endometrial carcinoma in relapse in 1985
Species: human (Homo sapiens)
Tissue: endometrium  
Disease: endometrium carcinoma
Gender: N/A 
Morphology: epitheloid cells growing in monolayers
Growth Mode: N/A 
Doubling Time: about 92 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI1640+10%FBS

EFE-184完全培养基,# CBP61196M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, NP
Cytogenetics: human flat-moded hypertriploid karyotype with 1-2 supernumerary dm in most cells and 23% polyploidy - 80(72-81)<3n>XX, -X, +1, +2, -4, -5, +6, +8, +10, -13, +17, +19, +20, +21, +6mar - der(1)t(1;?)(p11;?)x2, del(2)(q24)x2, der(2)t(2;3)(q11;p11), del(12)(p13), der(16)t(16;?)(q21/23;?), der(17)t(7;17)(q11;p11), add(22)(p11)x2
Virus   ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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