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HAL-01(人急性淋巴细胞白血病细胞)

CBP61018

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I. General information 
Synonyms: HAL-01
Background: established from the peripheral blood of a 17-year-old woman with acute lymphoblastic leukemia (ALL-L2) in 1990; contains the t(17;19)(q22;p13) TCF3-HLF (E2A-HLF) fusion gene
Species: human (Homo sapiens)
Tissue: N/A 
Disease: B cell precursor leukemia
Gender: N/A 
Morphology: single, round cells in suspension, some cells are slightly adherent; 
Growth Mode: N/A 
Doubling Time: ca. 48 hours
DNA Profile: N/A
Cobioer’s Cell Line Authentication Service
Culture Medium:

RPMI-1640+10%FBS

HAL-01完全培养基,# CBP61018M
We strongly suggest to purchase the complete medium from Cobioer.

Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD38 +, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; 
Fingerprint: fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics
Cytogenetics: human near-diploid karyotype with 4% polyploidy; 46(43-48)<2n>XX, t(1;7)(p34;q21), t(17;19)(q22;p13); t(17;19) effects rearrangement of TCF3 (E2A) and HLF; matches published karyotype
Molec. Genetics: expression of fusion gene TCF3-HLF (E2A-HLF) confirmed by RT-PCR
Virus   PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Comments: For more information, please contact Cobioer (4008-750-250).

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